Industry Standard Architecture (ISA) is the 16-bit internal bus of IBM PC/AT and similar computers based on the Intel 80286 and its immediate successors during the 1980s. The bus was (largely) backward compatible with the 8-bit bus of the 8088-based IBM PC, including the IBM PC/XT as well as IBM PC compatibles.

Originally referred to as the PC bus (8-bit) or AT bus (16-bit), it was also termed I/O Channel by IBM. The ISA term was coined as a retronym by IBM PC clone manufacturers in the late 1980s or early 1990s as a reaction to IBM attempts to replace the AT-bus with its new and incompatible Micro Channel architecture.

The 16-bit ISA bus was also used with 32-bit processors for several years. An attempt to extend it to 32 bits, called Extended Industry Standard Architecture (EISA), was not very successful, however. Later buses such as VESA Local Bus and PCI were used instead, often along with ISA slots on the same mainboard. Derivatives of the AT bus structure were and still are used in ATA/IDE, the PCMCIA standard, CompactFlash, the PC/104 bus, and internally within Super I/O chips.

Even though ISA disappeared from consumer desktops many years ago, it is still used in industrial PCs, where certain specialized expansion cards that never transitioned to PCI and PCI Express are used.